Redesigning the ubiquitin pathway to identify the substrates of E6AP
UBE3A function in the cell has remained elusive especially in terms of how UBE3A protein action relates to other proteins. Thus, researchers have been trying to identify “targets” (meaning proteins) that UBE3A is acting upon in order to either regulate their concentration or cause their degradation. This grant funded a unique method of providing intracellular protein tagging in hopes of identifying UBE3A targets and we learned from this project how difficult it is to identify such targets. This grant and others are part of an effort by ASF to identify UBE3A targets, increase understanding about UBE3A and promote new molecular therapeutic strategies.